Modified Postgate’s B Media (MPB)

Detection of Sulfate Reducing Bacteria (SRB)

What is MPB?

  • This highly sensitive SRB growth medium is currently the NACE TMO-194 standard for the cultivation of SRB in oil and gas systems
  • Modified Postgate’s B (MPB) Media was formulated by SRB research pioneer John Posgate in 1975
  • MPB is a strictly anaerobic media and is manufactured at BTS inside custom built anaerobic chambers

How It Works

  • MPB contains nutrients such as proteins, organic acids, and sulfate specially formulated to facilitate the growth of oil and gas field SRB
  • The redox potential of properly made MPB, typically less than -300mV, will allow for the reduction of the sulfate to sulfide by SRB
  • The biogenic sulfide created by SRB then reacts with dissolved iron species in the MPB solution. This reaction precipitates a black iron sulfide precipitate. Black precipitation in the vial indicates a positive for SRB

False Positive

If a water source inoculated into MPB has a significant concentration of dissolved sulfide, this can cause iron sulfide to form and create a false positive.